Dr. Shahnaz Babaei Abraki
Positions
Postdoctoral Associate
Calgary Foothills Primary Care Network
Background
Educational Background
Phd Developmental biology, University of Isfahan, 2022
Research
Areas of Research
We're developing techniques to expand both mouse and human pluripotent stem cells, such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), in stirred suspension bioreactors. These bioreactors offer several advantages over traditional static culture setups, including better control over conditions and higher cell yields. By maintaining physiological levels of key parameters and enabling large-scale growth in a short time, bioreactors minimize human intervention and reduce batch variability, making them crucial for stem cell applications. We've shown that the quality of cells expanded in bioreactors matches or exceeds that of cells expanded using static methods. However, when attempting to differentiate mouse ESCs in bioreactors, we encountered a significant difference: while differentiation was efficient in static culture, many cells in suspension remained pluripotent, hindering tissue differentiation. In mouse models, we've demonstrated that iPSCs form 1000-fold more efficiently and in half the time in bioreactors compared to static culture. This efficiency has allowed us to streamline the reprogramming process by removing certain genes. Our current research focuses on understanding how fluid shear influences cellular reprogramming through mechanotransduction.
Participation in university strategic initiatives
Awards
- Post-Doctoral Scholarship, Cumming School of Medicine. 2022
- Funding Fellowship Program, Eyes High Postdoctoral . 2022
Publications
- Ferroptosis: The functions of Nrf2 in human embryonic stem cells. (2022)
- Monitoring the induction of ferroptosis following dissociation in human embryonic stem cells. (2022)
- The Role of Endoplasmic Reticulum and Mitochondria in Maintaining Redox Status and Glycolytic Metabolism in Pluripotent Stem Cells. (2022)
- Astrocyte-mediated inflammation in cortical spreading depression. (2018)
- Stable knockdown of adenosine kinase by lentiviral anti-ADK miR-shRNAs in Wharton’s jelly stem cells. (2018)
- TRPV1 receptors augment basal synaptic transmission in CA1 and CA3 pyramidal neurons in epilepsy. (2017)
- Time course study of Aβ formation and neurite outgrowth disruption in differentiated human neuroblastoma cells exposed to H2O2: protective role of autophagy. (2013)
- Simultaneous inhibition of COX-2 and activation of PPAR-γ resulted in the same level and pattern of neuroprotection as they were targeted separately. (2013)
- Increase of autophagy and attenuation of apoptosis by Salvigenin promote survival of SH-SY5Y cells following treatment with H2O2. (2012)
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